Fluorescence-based evaluation of shRNA efficacy
نویسندگان
چکیده
منابع مشابه
An image-based, dual fluorescence reporter assay to evaluate the efficacy of shRNA for gene silencing at the single-cell level [version 2; referees: 2 approved]
RNA interference (RNAi) is widely used to suppress gene expression in a specific manner. The efficacy of RNAi is mainly dependent on the sequence of small interfering RNA (siRNA) in relation to the target mRNA. Although several algorithms have been developed for the design of siRNA, it is still difficult to choose a really effective siRNA from among multiple candidates. In this article, we repo...
متن کاملNanobiosensors and fluorescence based biosensors: An overview
A biosensor can sense biological elements after interaction with the recognition element. The signal produced due to interaction of the analyte with its biochemical element is transduced by a transducer and detected by appropriate modes. The miniaturization of these biosensors at the nano level using nanostructures as a platform for sensing the analyte or its detection is called a nanobiosensor...
متن کاملAn image-based, dual fluorescence reporter assay to evaluate the efficacy of shRNA for gene silencing at the single-cell level
RNA interference (RNAi) is widely used to suppress gene expression in a specific manner. The efficacy of RNAi is mainly dependent on the sequence of small interfering RNA (siRNA) in relation to the target mRNA. Although several algorithms have been developed for the design of siRNA, it is still difficult to choose a really effective siRNA from among multiple candidates. In this article, we repo...
متن کاملNanobiosensors and fluorescence based biosensors: An overview
A biosensor can sense biological elements after interaction with the recognition element. The signal produced due to interaction of the analyte with its biochemical element is transduced by a transducer and detected by appropriate modes. The miniaturization of these biosensors at the nano level using nanostructures as a platform for sensing the analyte or its detection is called a nanobiosensor...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2011
ISSN: 0003-2697
DOI: 10.1016/j.ab.2011.06.008